Metabolomic Analysis and Identification of Sperm Freezability-Related Metabolites in Boar Seminal Plasma

Sperm transcriptome profiling for assessment of boar semen freezability

In this project transcriptome analysis on highthroughput RNA-sequencing (RNA-Seq) data of boar spermatozoa will be utilized to identify novel genetic markers associated with semen freezability. Three boars each with poor and good semen freezability will be subjected to transcriptome studies on sperm RNA-seq data, and in combination with bioinformatics screening, will select candidate genes asso...

Proteomics of boar seminal plasma

Boar seminal plasma exosomes maintain sperm function by infiltrating into the sperm membrane

Seminal plasma ingredients are important for maintenance of sperm viability. This study focuses on the effect of boar seminal plasma exosomes on sperm function during long-term liquid storage. Boar seminal plasma exosomes had typical nano-structure morphology as measured by scanning electron microscopy (SEM) and molecular markers such as AWN, CD9 and CD63 by western blot analysis. The effect on...

mRNA profiling for the identification of sperm and seminal plasma

The influence of macro- and microelements in seminal plasma on diluted boar sperm quality

BACKGROUND Growing evidence indicates that macro- and microelements in the seminal plasma of humans and various domestic animals are of great importance due to their roles in sperm metabolism, function, survival and oxidative stress. In the present study, we therefore determined the concentrations of macro- and microelements in fresh boar seminal plasma and their relation to sperm quality param...